A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
DOI: 10.1126/science.1225829
PubMed: PMID 22745249
What this paper investigates
This foundational experimental study investigated how CRISPR-associated Cas9 uses guide RNAs to recognize and cleave specific DNA sequences. It helped establish the molecular basis for programmable genome editing.
Study approach
Biochemical experiments characterized RNA-guided Cas9 DNA cleavage and the role of guide-RNA components.
What to keep in mind
The paper establishes a molecular mechanism; it does not by itself demonstrate the safety or effectiveness of genome editing in patients.
Read the original research
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Citation information
Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. DOI: 10.1126/science.1225829